Biotin for Interference Testing (Solution) Catalog # K2010008B Lot # Batch Dependent Expiration Batch Dependent Volume 1 mL Appearance Clear solution Concentration 200 µg/mL in Diluent A Molar Mass 244.31 g/mol Storage –20°C or lower; aliquot to avoid freeze-thaw Other Names Biotin, vitamin H, vitamin B7, coenzyme R This biotin solution (200 µg/mL) is formulated for interference testing in immunoassays and biochemical platforms. Supplied in 1 mL aliquots and stabilized in Diluent A, the clear solution enables researchers to simulate biotin-related assay interference and assess robustness under physiologically relevant concentrations. Biotin is a known interferent in streptavidin-biotin based immunoassays, where excess biotin can displace assay components and generate false results. Applications include method validation, QC benchmarking, and interference panel construction. The solution is filtered for clarity and consistency, and should be aliquoted upon receipt to avoid repeated freeze-thaw cycles. This product is ideal for evaluating assay susceptibility to biotin interference and for developing mitigation strategies in diagnostic platforms, especially those involving sandwich or competitive formats. Key benefits: Biotin solution for interference testing in immunoassays 200 µg/mL concentration enables robust spiking protocols Filtered and stabilized in Diluent A Supports streptavidin-biotin assay formats Stable at –20°C; aliquot recommended Indication for Use: This product is for Research Use Only (RUO). This product is not for administration to humans or animals. This product is not for human or veterinary diagnostic or therapeutic use. All Interference Products Brochure Download PDF References: Schiettecatte J et al. Interferences in Immunoassays. Adv Immunoassay Tech. 2012. Miller JJ, Levinson SS. Interferences In Immunoassays. Immunoassay. 1996;165–190. Ward G. Immunoassay interference investigations. Pathology. 2013;45. Park JY, Kricka LJ. Immunoassay interference. Immunoassay Handbook. 2013;403–416. EP07: Interference Testing in Clinical Chemistry. CLSI. 2020. Gilles A et al. Targeting the 80S ribosome in cancer. Cells. 2020;9(3):E629. Coussens NP et al. Assay interference in proximity assays. Assay Guidance Manual. 2020. Wang L et al. Fluorescent probes for nitric oxide. Org Biomol Chem. 2020;18(8):1522–1549. Kawasaki H et al. Drosophila GBA mutant phenotypes. Gene. 2017;614:49–55. Hull B. HAMA interference with TSH assay. J S C Med Assoc. 2012;108(1):12–13. Degn SE et al. Rat kappa light-chain interference. J Immunol Methods. 2011;372(1–2):204–8.
