BMEM Max Pluripotent, comprising of BMEM Max Basal Medium and Primed Supplement, is a highly optimized, chemically defined, animal product-free basal formula for the maintenance and expansion of human induced pluripotent stem cells (hiPSCs) in adherent culture. BMEM Max Basal Medium is a new formula based on the rigorously optimized published formulation1 with modifications to allow for enhanced cell survival under suboptimal conditions such as single cell cloning for CRISPR modification, overgrowth, reduced media change and weekend-free protocols. Primed Supplement is new supplement specifically optimized for compatibility with adaptation of hiPSCs from legacy media such as mTeSR1™, a common problem with chemically defined media. Primed Supplement is optimized for component stability and to allow room temperature shipment. Culture of hiPSCs in BMEM Max Pluripotent results in increased cell proliferation and upregulation of core primed pluripotency factors when compared to legacy formulae1. BMEM Max Pluripotent is formulated for compatibility with weekend-free and no-change feeding schedules protocols. BMEM Max Pluripotent is compatible with a variety of culture matrices, including Corning® Matrigel® GFR, Gibco™ Geltrex™ GFR, R&D Systems™ Cultrex™ UltiMatrix GFR, SigmaAldrich® ECMatrix™-511 E8 Laminin, Gibco™ Recombinant Vitronectin, Corning® Synthemax II-SC. We highly recommend the use of BMEM Max Pluripotent with animal component-free matrices such as laminin 521 or Synthemax. BMEM Max Pluripotent is compatible with a variety of passaging methods including BMEM Dissociation (1002), Gibco™ TrypLE™, Stemcell Technologies™ ReLeSR™, Gibco™ Versene and Accutase. Each lot of BMEM Max and Primed Supplement is performance tested on hiPSC and tested for sterility. Larger unit sizes are available on request.Contains HEPES.
