Casein Blocking Buffer 10X Solution Catalog number: B2010111 Lot number: Batch Dependent Expiration Date: Batch dependent Volume/Weight: 50 mL pH: 7-7.4 Supplied as: 10X concentrate (dilute to 1X with di-water before use) Appearance: Slightly cloudy solution Applications: blocker for ELISA, Northern and Southern blotting. Storage: Room temperature Keywords: Casein blocking buffer, casein buffer for ELISA, casein blocking buffer for blotting Grade: Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um. References Ge M, Li RC, Qu T, Gong W, Yu XL, Tu C. Construction of an HRP-streptavidin bound antigen and its application in an ELISA for porcine circovirus 2 antibodies. AMB Express. 2017 Sep 18;7(1):177. doi: 10.1186/s13568-017-0473-3. PMID: 28921455; PMCID: PMC5603472.2: Poolperm P, Varinrak T, Kataoka Y, Tragoolpua K, Sawada T, Sthitmatee N. Development and standardization of an in-house indirect ELISA for detection of duck antibody to fowl cholera. J Microbiol Methods. 2017 Nov;142:10-14.3: Panda R, Boyer M, Garber EAE. A multiplex competitive ELISA for the detection and characterization of gluten in fermented-hydrolyzed foods. Anal Bioanal Chem. 2017 Dec;409(30):6959-6973. Jadhav PD, Shim YY, Reaney MJ. Synthesis and Characterization of Site- Selective Orbitide-BSA Conjugate to Produce Antibodies. Bioconjug Chem. 2016 Oct 19;27(10):2346-2358.5: Kim WJ, Cho HY, Jeong B, Byun S, Huh J, Kim YJ. Synergistic Use of Gold Nanoparticles (AuNPs) and “Capillary Enzyme-Linked Immunosorbent Assay (ELISA)” for High Sensitivity and Fast Assays. Sensors (Basel). 2017 Dec 26;18(1):55.
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