ELISA Blocking Buffer (10X Solution) Catalog number: B2010010 Lot number: Batch Dependent Expiration date: Batch Dependent Volume: 100 mL Appearance: Clear to slightly white Solution Application: ELISA Shelf-Life 3 years Storage: Room temperature Keywords: Elisa blocking protocol, ELISA blocking buffer, blocking Elisa plates, blocking buffer Elisa. References 1: Enzyme immunoassay and enzyme-linked immunosorbent assay. Gan SD, Patel KR. J Invest Dermatol. 2013 Sep;133(9):e12. 2: Enzyme-linkedimmunosorbentassay (ELISA). Reen DJ. Methods Mol Biol. 1994;32:461-6. 3: Recent Advances in Enhancement Strategies for Electrochemical ELISA-Based Immunoassays for Cancer Biomarker Detection. Arya SK, Estrela P. Sensors (Basel). 2018 Jun 22;18(7):2010. 4: The application of immunoassay techniques, including enzyme-linkedimmunosorbentassay (ELISA), to snake venom research. Theakston RD. Toxicon. 1983;21(3):341-52. 5: Commercial enzyme-linked immunosorbent assay versuspolymerase chain reaction for the diagnosis of chronic Chagas disease: a systematic review and meta-analysis. Brasil PE, Castro R, Castro Ld. Mem Inst Oswaldo Cruz . 2016 Jan;111(1):1-19. 6: Lequin RM. Enzyme immunoassay (EIA)/enzyme-linked immunosorbent assay (ELISA). Clin Chem. 2005 Dec;51(12):2415-8. 7: Aydin S. A short history, principles, and types of ELISA, and our laboratory experience with peptide/protein analyses using ELISA. Peptides. 2015 Oct;72:4-15. 8: Eble JA. Titration ELISA as a Method to Determine the Dissociation Constant of Receptor Ligand Interaction. J Vis Exp. 2018 Feb 15;(132):57334. 9: Pang B, Zhao C, Li L, Song X, Xu K, Wang J, Liu Y, Fu K, Bao H, Song D, Meng X, Qu X, Zhang Z, Li J. Development of a low-cost paper-based ELISA method for rapid Escherichia coli O157:H7 detection. Anal Biochem. 2018 Feb 1;542:58-62. 10: Major M, Law M. Detection of Antibodies to HCV E1E2 by Lectin-Capture ELISA. Methods Mol Biol. 2019;1911:421-432. doi: 10.1007/978-1-4939-8976-8_28.
