Core Direct qRT-PCR TaqProbe Kit

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The Core Direct qRT-PCR TaqProbe Kit is designed for real-time quantitative analysis of target RNA directly from blood, serum/plasma, swabs, cell cultures, bacterial colonies, and animal or plant tissue. The kit enables robust amplification without requiring prior RNA purification procedures. The kit is optimized with our unique inhibitor-resistant, thermostable polymerases and buffer system for optimal performance. In most cases, samples can be added directly to the RT-PCR master mix without the need for pre-lysis. The blend of polymerases is packaged separately from the 2X RT-PCR Mix, allowing scientists to optimize the enzyme amount for their specific application based on the inhibitory level in the samples. The kit ensures fast and easy preparation with a minimum of pipetting steps and is especially recommended for: Direct detection of viral or bacterial RNA in nasal or throat swabs Direct qRT-PCR from blood samples and other body fluid Direct qRT-PCR from cell culture Direct quantitative analysis of target RNA from single colonies or cells Direct amplification of target RNA from various tissue samples Highlight Unique Invention: First One-Step Direct qRT-PCR system able to tolerate high inhibitors in the market Time saving: No need for RNA extraction, one-step, performance of RT and PCR in same reaction Convenient: Only need adding enzymes, primers, probe and sample into 2X mix High inhibitor-resistance: Can amplify RNA target in reaction containing high level of PCR inhibitors Data Sheet: Core Direct qRT-PCR TaqProbe Kit Show more