The Core Direct RT-PCR Kit is designed to analyze target RNA directly from blood, serum/plasma, swabs, cell cultures, bacterial colonies, and animal or plant tissue. The kit enables robust amplification without requiring prior RNA purification procedures. The kit is optimized with our unique inhibitor-resistant, thermostable polymerases and buffer system for optimal performance. In most cases, samples can be added directly to the RT-PCR master mix without the need for pre-lysis. The blend of polymerases is packaged separately from the 2X RT-PCR Mix, allowing scientists to optimize the enzyme amount for their specific application based on the inhibitory level in the samples. The kit ensures fast and easy preparation with a minimum of pipetting steps and is especially recommended for: Direct detection of viral or bacterial RNA in nasal or throat swabs Direct amplification from blood samples and other body fluid Direct PCR from cell culture Direct detect RNA from colony or cells Direct amplification of target RNA from various tissue samples Point-of-Care diagnostics Highlight Unique Invention: First One-step Direct RT-PCR system able to tolerate high inhibitors in the market Time saving: No need for RNA extraction, one-step, performance of RT and PCR in same reaction Convenient: Only need adding enzyme, primers and sample in 2X mix High inhibitor-resistance: Can amplify RNA target in reaction containing high level of PCR inhibitors Versatile: Formulation works with blood, saliva, body fluids, other liquid samples and more Data Sheet: Core Direct RT-PCR Kit
