Description DNase I is an endonuclease that can digest single- or double-stranded DNA. It can hydrolyze phosphodiester bonds to produce mono- and oligodeoxynucleotides containing a 5'-phosphate group and a 3'-OH group.The optimal working pH range of DNase I is 7-8. The activity of DNase I depends on Ca2+ and can be activated by divalent metal ions such as Co2+, Mn2+, Zn2+, etc. In the presence of Mg2+, DNase I can randomly cleave any site of double-stranded DNA; while in the presence of Mn2+, DNase I can cleave DNA double-stranded at the same site, forming blunt ends or sticky ends with 1-2 nucleotides protruding.This enzyme is derived from recombinant E. coli strains, does not contain RNase, and can be used for the treatment of various RNA samples. Features Recombinant enzyme Stringent quality control Applications Preparation of DNA-free RNA Removal of template DNA following in vitro transcription Preparation of DNA-free RNA prior to RT-PCR and RT-qPCR DNA labeling by nick-translation in conjunction with DNA Polymerase I Studies of DNA-protein interactions by DNase I, RNase-free footprinting Specifications Expression Host Recombinant E. coli with Dnase I gene Purity ≥99% (SDS-PAGE) Storage Buffer 10 mM Tris-HCl (pH 7.6), 2 mM CaCl2, 50% glycerol Unit Definition The amount of enzyme required to completely degrade 1 μg of plasmid DNA at 37℃ for 10 mins Components Components No. Name 10325ES80 (1,000U) 10325ES90 (5,000 U) 10325-A Recombinant DNase I (RNase-free) -2 U/μL 500 μL 5×500 μL 10325-B DNase I Reaction Buffer (10×) 1 mL 5×1 mL Storage The product should be stored at -25℃ ~ -15℃ for two years. Please avoid repeated freeze-thaw. Documents: Safety Data Sheet 10325_MSDS_HB220421_EN_1652931562479.PDF 10325_MSDS_HB220421_EN_1652931562479.PDF Manuals 10325_Manual_HB230113_EN.pdf
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