Product Details Elevate your RNA isolation protocols with the EasyPure® RNA Kit, designed to deliver high-quality RNA from diverse biological samples, including animal cells, animal tissues, bacteria, and yeast. This advanced kit employs a column-based method that ensures rapid and efficient RNA extraction. The process begins with enzymatic lysis of cells and tissues, followed by DNase I treatment to eliminate DNA contamination. RNA is then selectively bound to a silica membrane, washed, and eluted in its pure form, free from protein contaminants. Features Contains DNase I which removes contaminating DNA from RNA samples. Safe and Non-Toxic: No need for phenol and chloroform. High Yield and Purity: Delivers RNA suitable for RT-PCR, qRT-PCR, and Northern blotting. Versatile: Effective for animal cells, tissues, bacteria, and yeast. See protocol details in manual: Sample requirements Material Amount Volume of BB4/β-ME Animal cells ≤5x10^6 0.3-0.6 ml Animal tissues ≤20 mg 0.3-0.6 ml Bacterial Cells ≤1x10^9 0.35 ml *BB4 with β-mercaptoethanol; add 10 μl of β-mercaptoethanol per 1ml of BB4, the solution must be freshly prepared just before use. Kit Content Not provided in kit: β-mercaptoethanol 96%-100% ethanol Component ER101-01(50 rxns) Binding buffer 4 (BB4) 40 ml Clean buffer 4 (CB4) 60 ml Wash buffer 4 (WB4) 12 ml Proteinase K (20 mg/ml) 1 ml DNase I (3 units/μl) 1500 units DNase I Reaction Buffer 4×1 ml RNase-free Water 10 ml RNase-free Tube (1.5 ml) 50 each RNA Spin Columns with Collection Tubes 50 each
