Introduction HiPure FFPE RNA Kit supplies a simple and rapid RNA extraction for Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 30 minutes (not including digestion time). RNA can be directly used for downstream applications such as RT-PCR, Northern blot, vitro translation and other experiments. Details Specifications Features Specifications Main Functions Isolation total RNA from FFPE tissue and section samples Applications RT-PCR, quantitative RT-PCR, Northern hybridization, Poly A purification, nucleic acid protection and in vitro translation Purification method Mini spin column Purification technology Silica technology Process method Manual (centrifugation or vacuum) Sample type FFPE tissue sample Sample amount 6mg Yield 20μg Elution volume ≥20μl Time per run ≤60 minutes Liquid carrying volume per column 800µl Binding yield of column 100µg Principle This product is based on silica column purification. Remove paraffin by Buffer DPS. Sample lysis with proteinase K digestion requires only 15 minutes. After lysis, samples are incubated at 80ºC for 15 minutes. Transfer to an adsorption column and RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, RNA was finally eluted with low-salt buffer. Advantages High quality - high purity total RNA can be directly used in various sensitive downstream applications Fast - several samples can be extracted in 60 minutes by column method Safe - no phenol chloroform extraction required Sensitive - RNA can be recovered at the level of PG Kit Contents Contents R414302 D414303 Purification Times 50 Preps 250 Preps HiPure RNA Micro Columns 50 250 2ml Collection Tubes 50 250 Buffer DPS 60 ml 250 ml Buffer FRL 15 ml 60 ml Buffer RLC 15 ml 60 ml Buffer RWC* 10 ml 50 ml Buffer RW2* 20 ml 2 x 50 ml Protease Dissolve Buffer 1.8 ml 10 ml Proteinase K 24 mg 120 mg RNase Free Water 10 ml 20 ml Storage and Stability Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
