Overview Product Description Recombinant Human IL-15 Protein is expressed from E.coli without tag.It contains Asn49-Ser162. Product Background Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases.IL-15 is a cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA. Product Category Recombinant Protein Specifications Target IL-15 Synonyms L15; IL-15; IL-15MGC9721; Interleukin-15 Gene/Protein ID P40933 Species Human Exact Sequence Asn49-Ser162 Molecular Weight The protein has a predicted MW of 12.8 kDa same as Tris-Bis PAGE result. Activity Immobilized Human IL-15 at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-15RA, His Tag with the EC50 of 22.1ng/ml determined by ELISA. The affinity constant of 5.9nM as determined in SPR assay (Biacore T200). See testing image for detail. Product Tag No Tag Express System E.coli Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC Endotoxin Less than 1EU per ug by the LAL method. Shipping, Reconstitution, & Storage Form Lyophilized Shipping Shipped at ambient temperature. Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Datasheet MSDS CoA
