Overview Product Description Recombinant Human IL-7 Protein is expressed from E.coli without tag.It contains Asp26-His177. Product Background Interleukin-7 (IL7) plays a nonredundant role in T cell survival and homeostasis, which is illustrated in the severe T cell lymphopenia of IL7-deficient mice, or demonstrated in animals or humans that lack expression of either the IL7Rα or γ c chain, the two subunits that constitute the functional IL7 receptor. Product Category Recombinant Protein Specifications Target IL-7 Synonyms IL7; Interleukin-7; IL-7; Lymphopoietin-1; PBGF Gene/Protein ID P13232 Species Human Exact Sequence Asp26-His177 Molecular Weight The protein has a predicted MW of 17.5 kDa same as Tris-Bis PAGE result. Activity Immobilized Human IL-7 at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 52.5ng/ml determined by ELISA. The affinity constant of 4.18 nM as determined in SPR assay (Biacore T200). See testing image for detail. Product Tag No Tag Express System E.coli Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC Endotoxin Less than 1EU per ug by the LAL method. Shipping, Reconstitution, & Storage Form Lyophilized Shipping Shipped at ambient temperature. Formulation Lyophilized from 0.22um filtered solution in 25mM PB, 250mM NaCl (pH 6.8). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 25mM PB, 250mM NaCl (pH 6.8). Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Datasheet MSDS CoA
