Product Specification: Item# 3501: Human Anti-gp120 HIV-1 Monoclonal Antibody Antibody Class: IgG Concentration: See vial Mass/vial: 100µg Purity: >95% Stabilizer: None Preservative: None Physical State: Frozen Liquid Storage: -75°C Stability: At least 12 months at -75°C Application: ELISA, Western ELISA, Immunoprecipitation, Immunohistochemistry, HIV-1 Neutralization, Description: High affinity Human anti-gp120 HIV-1 mAb. Human-mouse chimera produced in human myeloma cell line in culture. Specificity: Binds to CD4 binding region of gp120. Biological Activity: Neutralizes HIV-1 IIIB at 1-10µg/ml as determined by syncycium assays with CD4-bearing HIV-1 IIIB infected cells and co-cultures of infected PBLs. Application and Instructions for use: Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA may be performed at 10-100ng/ml of mAb depending on the amount of immobilized antigen in the assays. Western ELISA requires 1-5µg of mAb/ml. Neutralization assays are performed in 1-10µg/ml range depending on virus TCID. Caution: Avoid use of needles and ensure proper laboratory safety precautions are in place for the use of this product.
