PRODUCT SPECIFICATION Product# 3504: Human Anti-gp160 HIV-1 Monoclonal Antibody SPECIFICATIONS APPLICATIONS Antibody Class: IgG ELISA, Western ELISA Concentration: See vial Immunoprecipitation Mass/vial: 100µg Immunohistochemistry Purity: >95% HIV-1 Neutralization Stabilizer: None Preservative: None Physical State: Frozen Liquid Storage: -75oC Stability: At least 2 months at -750C. DESCRIPTION: High affinity Human anti-gp160 HIV-1 mAb. SPECIFICITY: Binds to CD4 binding region of gp160. BIOLOGICAL ACTIVITY: Neutralizes HIV-1 IIIB at 1-10µg/ml as determined by syncycium assays with CD4-bearing HIV-1 IIIB infected cells and co-cultures of infected PBLs. APPLICATIONS AND INSTRUCTIONS FOR USE Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA may be performed at 10-100ng/ml of mAb depending on the amount of immobilized antigen in the assays. Western ELISA requires 1-5µg of mAb/ml. Neutralization assays are performed in 1-10µg/ml range depending on virus TCID. CAUTION: Avoid use of needles and ensure proper laboratory safety precautions for the use of this product.
