Specification Package 2 KU/10 KU/5 X 10 KU Full name M-MuLV Reverse Transcriptase Applications First-strand cDNA synthesis for RT-PCR and real-time RT-PCR; synthesis of cDNA for cloning and expression studies; Preparation of labeled cDNA probes for microarray research; DNA marking; Primer extension method for RNA analysis Summary M-MuLV reverse transcriptase (RT) is a recombinant M-MuLV RT. It differs from M-MuLV RT in terms of structure and catalytic performance. The enzyme has RNA-dependent and DNA-dependent polymerase activity as well as RNase H activity specific to RNA in RNA-DNA hybrids, which is significantly lower than that of avian myeloblastomavirus (AMV) reverse transcriptase RNase H Minus and acts in a wide range of temperatures (42~55°C). Features Efficient synthesis of full-length first-strand cDNA up to 13 kb, with activity up to 50°C at 42°C and addition of modified nucleotides Composition M-MuLV Reverse Transcriptase (200U/ul), 5X Reaction Buffer Reaction Buffer 5X Reaction Buffer Unit Definition One unit is defined as the amount of enzyme that catalyzes the incorporation of 10 nmol dTMP into a polynucleotide fraction in 10 min at 37°C. Thermal Inactivation Heat at 70 °C for 10 min to lose activity. Concentration 200 U/μl Storage Buffer 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 1 mM EDTA, 5 mM DTT, 0.1% (v/v) Triton X-100, 50% (v/v) glycerin. Component M-MuLV Reverse Transcriptase (200U/ul), 5X Reaction Buffer Storage -20°C
