/* ========================= Biofargo Table SOP (Embedded) ========================== */ .biofargo-wrap .biofargo-table-wrap{ overflow-x:auto; -webkit-overflow-scrolling:touch; border:1px solid #eee; border-radius:10px; padding:6px; background:#fff; margin-top:15px; } .biofargo-wrap table.biofargo-table{ width:100%; border-collapse:collapse; font-size:14px; min-width:640px; background:#fff; } .biofargo-wrap table.biofargo-table th, .biofargo-wrap table.biofargo-table td{ border:1px solid #e9ecef; padding:15px; vertical-align:middle; white-space:normal; word-break:break-word; } .biofargo-wrap table.biofargo-table thead th{ background:#f2f2f2; color:#000; font-weight:700; text-align:left; } .biofargo-wrap table.biofargo-table tbody td:first-child{ background:#f8f9fa; font-weight:600; width:30%; } Description M-MuLV reverse transcriptase (RT) is a recombinant M-MuLV RT. It differs from M-MuLV RT in terms of structure and catalytic performance. The enzyme has RNA-dependent and DNA-dependent polymerase activity as well as RNase H activity specific to RNA in RNA-DNA hybrids, which is significantly lower than that of avian myeloblastomavirus (AMV) reverse transcriptase RNase H Minus and acts in a wide range of temperatures (42~55°C). Specification Full name M-MuLV Reverse Transcriptase Reaction Buffer 5X Reaction Buffer Unit Definition One unit is defined as the amount of enzyme that catalyzes the incorporation of 10 nmol dTMP into a polynucleotide fraction in 10 min at 37°C. Thermal Inactivation Heat at 70 °C for 10 min to lose activity. Concentration 200 U/μl Storage Buffer 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 1 mM EDTA, 5 mM DTT, 0.1% (v/v) Triton X-100, 50% (v/v) glycerin. Features Efficient synthesis of full-length first-strand cDNA up to 13 kb, with activity up to 50°C at 42°C and addition of modified nucleotides Applications First-strand cDNA synthesis for RT-PCR and real-time RT-PCR synthesis of cDNA for cloning and expression studies Preparation of labeled cDNA probes for microarray research DNA marking Primer extension method for RNA analysis Components M-MuLV Reverse Transcriptase (200U/ul), 5X Reaction Buffer Storage -20°C
