Mix MaestroSafe loading dye directly with your sample! No need to add any dyes to both gel matrix and running buffers! Works with both blue light and UV transilluminators! MaestroSafeTM products represent a new and safe class of nucleic acid stains for visualization of double-stranded DNA , single-stranded DNA , and RNA in agarose gels. The dyes are developed to replace toxic Ethidium Bromide (EtBr , a potent mutagen) , commonly used in gel electrophoresis for visualization of nucleic acids in agarose gels. With MaestroSafe dyes , you do not need to add any dyes to both gel matrix and running buffers.MaestroSafe dyes are provided in a form of 6X sample loading dyes and they are to be added to your samples only. After the electrophoresis , view and document your results as you would do with EtBr staining protocols. Protocol:1.Prepare a 100ml agarose solution.2.Mix gentle without having any air bubbles.3.Let the solution cool down to 60-70℃ and cast the gel.4.Mix samples and DNA markers with a MaestroSafeTM dye at 1:10 dilution rate.5.View the results under traditional UV light after electrophoresis.6.Or use non -hazardous UltraBright or UltraSlim LED transilluminator Reference: MaestroSafeTM was developed to work with Blue Light LED illuminators (like UltraSlim LED and UltraBright LED) but it can also be used with regular UV transilluminators as well. The excitation wavelength peak is ~470nm. Emission peak is ~530 nm. Safety: MaestroSafe stain is non-carcinogenic and less mutagenic compared to Ethidium Bromide. MaestroSafe stain is impenetrable to latex gloves and cell membranes. MaestroSafe stain is classified as non-hazardous to aquatic life, under CCR Title 22 regulation. Please see the detailed safety report here. Better results in downstream application:Better cloning efficiency for “MaestroSafe stain + UltraBright LED” compared with “Ethidium Bromide + UV transilluminator" Storage and shipping information: Shipping temperature :The delivery in room temperature will be alright. (25~30℃). MaestroSafe loading dye can be stored in room temperature as long as three weeks without any bad influence on it. Storage conditions : Our kind suggestion would be stored under 4℃ for long-term storage. Shelf-life : Store at 4°C up to 24 months. MaestroSafeTM products are non-carcinogenic by the Ames-test. The results are negative in both the mouse marrow chromophilous erythrocyte micronucleus and mouse spermary spermatocyte chromosomal aberration tests.
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