PRODUCT SPECIFICATION Product# 1191: Murine anti-gp41 HIV-1 Monoclonal Antibody SPECIFICATIONS APPLICATIONS Antibody Class: IgG1 ELISA, Western ELISA Concentration: 1mg/ml Immunoprecipitation Mass/vial: 100µg/vial Immunocytochemistry Diluent: 20mM NaPO4, 10mM NaCl, pH7 Immunohistochemistry Purity: >95% Stabilizer: None Preservative: None Physical State: Frozen Liquid Storage: -75oC Stability: At least 24 months at -75oC. DESCRIPTION: High affinity murine anti-gp41 mAb. SPECIFICITY: Binds to native and recombinant gp41 HIV-1 in Elisa and Western Elisa. Epitope mapped to amino acids 730 - 750. BIOLOGICAL ACTIVITY: Not determined. APPLICATIONS AND INSTRUCTIONS FOR USE Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA may be performed at 10-100ng/ml of mAb depending on the amount of immobilized antigen in the assays. Western ELISA requires 1-5µg of mAb/ml. RELATED PRODUCTS PRODUCT#: 1191-P Peroxidase-Conjugated Murine anti-gp41HIV-1 Application: ELISA, Western ELISA, Histocytochemistry, Histocytology. PRODUCT#: 1191-F Fluorescene Murine anti-gp41 HIV-1 Application: FACS, Cell Staining PRODUCT#: 1191-G Colloidal Gold-Conjugated Murine anti-gp41 HIV-1 Application: Dot Blots, EM.