PRODUCT SPECIFICATION Product# 1105: Murine anti-p17 HIV-1 Monoclonal Antibody SPECIFICATIONS APPLICATIONS Antibody Class: IgG1 ELISA, Western ELISA Concentration: See vial Immunoprecipitation Mass/vial: 100µg Immunocytochemistry Purity: >95% Immunohistochemistry Stabilizer: None Diagnostics Preservative: None Physical State: Liquid Storage: -75oC Stability: At least 12 months at 75oC. DESCRIPTION: High affinity murine anti-p17 HIV-1 mAb. SPECIFICITY: Reactive with recombinant and native p17 HIV-1 and HIV-1 infected cultures. Also reactive with HIV-1 viral lysates in Western blots. Immunoprecipitates p55 gag protein. BIOLOGICAL ACTIVITY: Not Determined.. APPLICATIONS AND RECOMMENDATIONS FOR USE Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA may be performed at 10-100ng of mAb depending on the amount of immobilized antigen in the assays. Western ELISA and indirect immunofluorescence studIEs with HIV-1 infected cells may be performed with 1-5µg/ml of mAb. RELATED PRODUCTS PRODUCT#: 1105-P Peroxidase-Conjugated Murine anti-p17 HIV-1 IIIB Application: ELISA, Western ELISA, Histocytochemistry, Histocytology. PRODUCT#: 1105-F Fluorescene Murine anti-p17 HIV-1 IIIB Application: Cell Staining PRODUCT#: 1105-G Colloidal Gold-Conjugated Murine anti-p17 HIV-1 IIIB Application: Dot Blots, EM.
