PRODUCT SPECIFICATION Product# 1108: Murine HIV-1 anti-nef Monoclonal Antibody SPECIFICATIONS APPLICATIONS Antibody Class: IgG1 ELISA, Western ELISA Concentration: See vial Immunoprecipitation Mass/vial: 100µg Immunohistochemistry Purity: >95% Diagnostics Stabilizer: None Preservative: None Physical State: Frozen Liquid Storage: -75oC Stability: At least 12 months at -75oC DESCRIPTION: High affinity murine anti-nef HIV-1 (LAV) mAb. SPECIFICITY: Binds to native and recombinant nef in ELISA and Western ELISA. BIOLOGICAL ACTIVITY: Not Determined. APPLICATIONS AND INSTRUCTIONS FOR USE Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA may be performed at 10-100ng/ml of mAb depending on the amount of immobilized antigen in the assays. Western ELISA requires 1-5µg of mAb/ml.
