Introduction HEPES Buffer 1 M is a ready-to-use, highly concentrated buffer solution designed for direct use in cell culture research applications. HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic sulfonic acid buffering agent; one of the twenty Good's buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by aerobic respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. A GMP Grade version is now available: PAN-Biotech HEPES Buffer 1 M, GMP Grade. Composition HEPES 238.31 g/L Specifications Cat. No. P05-01100, P05-01500 Quality: Research Use Only (RUO) Liquid / Powder: Liquid Product Category: Media supplements Product Type: HEPES Size: 100 ml or 500 ml bottles Endotoxin: