Description PBS Solution (Phosphate Buffered Saline) is supplied as a 500 mL 10X concentrated Cell Culture Grade buffer recommended for immunohistology protocols. The concentrated formulation allows preparation of up to 5 L of 1X PBS working solution for routine laboratory workflows. The 1X PBS formulation contains 137 mM Sodium Chloride, 2.7 mM Potassium Chloride and 10 mM Phosphate Buffer with a pH of 7.4–7.6 at 25°C. The solution is sterile and tested free of detectable DNase, RNase and protease activity, making it suitable for cell culture, ELISA, Western blotting, immunological assays and molecular biology workflows. Disclaimer: For Laboratory Use Only. Not for drug, household, human or veterinary use. Specification Chemical Name PBS Solution (Phosphate Buffered Saline) Appearance Liquid Grade Cell Culture pH 7.3–7.5 Sterility Pass DNase None Detected RNase None Detected Protease None Detected Storage 18–25 °C Features 10X Concentrated FormulationSupplied as a concentrated formulation that can be diluted 1:10 to prepare 1X PBS working solution. 500 mL Package Size Each bottle prepares up to 5 liters of 1X PBS for routine laboratory applications. Cell Culture Grade Quality Manufactured and tested to Cell Culture Grade specifications. Sterility Verified Tested and confirmed to pass sterility requirements. DNase-, RNase-, and Protease-Free No detectable nuclease or protease activity for sensitive biological workflows. Physiological pH Buffer System Formulated to provide pH 7.4–7.6 at 25°C for biological applications. Recommended for Immunohistology Specifically recommended for immunohistology protocols according to manufacturer documentation. Applications Cell Culture Workflows — Cell washing, media preparation, sample dilution and maintenance of physiological conditions. ELISA Procedures — Washing steps, reagent dilution and assay preparation. Western Blot Applications — Membrane washing and antibody incubation workflows. Immunohistology & Immunohistochemistry (IHC) — Tissue washing, staining and sample preparation procedures. Molecular Biology Research — DNA, RNA and protein workflows requiring sterile nuclease-free buffer systems. General Laboratory Use — Routine washing, dilution and buffer preparation applications. Documents INFO COA SDS Frequently Asked Questions (FAQ) What is the dilution ratio of this 10X PBS Solution? This PBS Solution is supplied as a 10X concentrate and should be diluted 1:10 with sterile water to prepare 1X working solution. How much 1X PBS can be prepared from one 500 mL bottle? One 500 mL bottle of 10X PBS can prepare up to 5 liters of 1X PBS working solution. Is this PBS Solution sterile? Yes. The product is tested and confirmed to meet sterility requirements. Is this PBS DNase-free, RNase-free and protease-free? Yes. Testing confirms that DNase, RNase and protease activity are not detected. What is the pH of the 1X PBS solution? The pH of the 1X PBS solution is 7.4–7.6 at 25°C. Can this PBS be used for ELISA and Western blot applications? Yes. PBS Solution is suitable for ELISA washing procedures, Western blot workflows, immunological assays and other routine laboratory applications. How should this PBS Solution be stored? Store at 18–25°C in a tightly closed container in a dry and well-ventilated area. 10x pbs 10x pbs solution 10x phosphate buffered saline 500ml pbs 500 ml pbs solution cell culture pbs sterile pbs dnase free pbs rnase free pbs protease free pbs pbs wash buffer elisa wash buffer western blot wash buffer ihc wash buffer immunohistology buffer ph 7.4 pbs phosphate buffered saline 10x molecular biology pbs laboratory pbs buffer
