This vector contains enhanced cyan fluorescent protein - ECFP- driven by cytomegalovirus (CMV) promoter for high level expression in mammalian cells. The multiple cloning site (MCS) is located at both N1 and C1 terminus. Plasmid carries ampicillin resistance for bacterial selection and neomycin resistance for stable selection with G418 in eukaryotic cells. Total vector size 6107 bp. Research Use Only (RUO). Applications Mammalian cell transfection ECFP fusion protein expression Fluorescence imaging Protein localization studies Live-cell microscopy Gene expression analysis Cell biology research Molecular cloning Features Strong CMV promoter for high-level expression Enhanced Cyan Fluorescent Protein (ECFP) reporter Suitable for transient and stable expression studies Ideal for imaging and localization experiments