pEASY®-Blunt E2 Expression Kit

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Features: • 5 minutes fast ligation of Pfu-amplified PCR products. • Ampicillin resistance. • T7 promoter primer and T7 terminator primer for sequencing. • Bacteriophage T7lac prom oter for high level expression. • C-terminal 6×His tag for easy purification. • Trans1-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing. • E2 Expression Plasmid included as negative control. Storage Chemically Competent Cell at -70°C for six months; others at -20°C for nine months Kit Content Component CE211-01 (10 rxns) pEASY®-Blunt E2 Expression Vector (15 ng/μl) 10 µl E2 Expression Plasmid (Negative Control) (15 ng/μl) 10 µl EControl Template (5 ng/μl) 10 µl EControl Forward Primer (10 μM) 10 µl EControl Reverse Primer (10 μM) 10 µl T7 Promoter Primer (10 μM) 50 µl T7 Terminator Primer (10 μM) 50 µl Trans1-T1 Phage Resistant Chemically Competent Cell 5×100 μl Paper citing this product: Literature Journal IF Author Date Link A Novel NHERF1 Mutation in Human Breast Cancer and Effects on Malignant Progression. anticancer research 1.9 Xiaomei Yang Shan Cheng, Department of Biochemist 2017 Jan The original link Show more