Features: • 5 minutes fast ligation of Pfu-amplified PCR products. • Enhanced CMV promoter for higher protein yield. • CMV forward primer and TK polyA reverse primer for sequencing. • C-terminal myc tag for protein detection. • Neomycin resistance gene for stable cell line selection. Trans1-T1 phage resistant chemically competent cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing. • M2 Expression Plasmid included as negative control. Storage Chemically Competent Cell at -70°C for six months; others at -20°C for nine months Kit Content: Component CM211-01 (10 rxns) pEASY ®-Blunt M2 Expression Vector (15 ng/μl) 10 µl M2 Expression Plasmid (Negative Control) (15 ng/μl) 10 µl MControl Template (5 ng/μl) 10 µl MControl Forward Primers (10 μM) 10 µl MControl Reverse Primers (10 μM) 10 µl CMV Forward Primer (10 μM) 50 µl TK PolyA Reverse Primer (10 μM) 50 µl Trans1-T1 Phage Resistant 5×100 μl Chemically Competent Cell Papers citing the use of this product: Literature Journal IF Author Date Link The cellular distribution of Na+/H+ exchanger regulatory factor 1 is determined by the PDZ-I domain and regulates the malignant progression of breast cancer Oncotarget 5.008 Du G, et al. Department of Biochemistry and Molecu 2016 May The original link A Novel NHERF1 Mutation in Human Breast Cancer and Effects on Malignant Progression anticancer research 1.895 Yang X, et al. Department of Biochemistry and Mole 2017 Jan The original link A Novel NHERF1 Mutation in Human Breast Cancer Inactivates Inhibition by NHERF1 Protein in EGFR Signaling Anticancer Research 1.826 GUIFANG DU, et al. 2016 Mar The original link
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