Description pEASY ®-Blunt Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain non-recombinant vector are killed upon plating. Therefore, blue/white selection is not required. •5 minutes fast ligation of Pfu-amplified PCR products. •High cloning efficiency. Positive clones up to 100%. •No blue/white selection needed. •Suitable for larger fragment cloning. •Kanamycin and Ampicillin resistance genes for selection. •M13 forward primer and M13 reverse primer for sequencing. •T3 promoter and T7 promoter for in vitro transcription. Storage At -20°C for 9 months Kit Content Component CB501-01 CB501-02 (20 rxns) (60 rxns) pEASY ®-Blunt Zero Cloning Vector (10 ng/μl) 20 µl 3×20 µl Control Template (5 ng/μl) 5 µl 5 µl Control Primers (10 μM) 5 µl 5 µl M13 Forward Primer (10 μM) 50 µl 150 µl M13 Reverse Primer (10 μM) 50 µl 150 µl Please note: this kit does not contain competent cells. References: Literature Journal IF Author Date Link NdhV is a subunit of NADPH dehydrogenase essential for cyclic electron transport in Synechocystis sp. strain PCC 6803 plant physiology 6.28 Gao F, et al. College of Life and Environment Scie 2016 Feb The original link Regulation of DNA phosphorothioate modification in Salmonella enterica by DndB. Scientific Reports 5.228 He W, et al. Key Laboratory of Combinatorial Biosy 2015 Jul The original link Proline accumulation and metabolism-related genes expression profiles in Kosteletzkya virginica seedlings under salt stress FRONTIERS IN PLANT SCIENCE 4.495 Wang H, et al. Key Laboratory of Coastal Biology a 2015 Sep The original link CRISPR/Cas9-mediated targeted mutagenesis in Nicotiana tabacum plant molecular biology 3.905 Gao J, et al. State Key Laboratory of Silkworm Gen 2015 Jan The original link The Different Potential of Sponge Bacterial Symbionts in N2 Release Indicated by the Phylogenetic Diversity and Abundance Analyses of Denitrification Genes, nirK and nosZ PLoS One 3.73 Zhang X, et al. Marine Biotechnology Laboratory, S 2013 Jun The original link
