Description pEASY ®-T5 Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain non-recombinant vector are killed upon plating. Therefore, blue/white screening is not required. •5 minutes fast ligation of Taq-amplified PCR products. •High cloning efficiency. Positive clones up to 100%. •No blue/white selection needed. •Suitable for short and large fragment cloning. •Kanamycin and Ampicillin resistance genes for selection. •M13 forward primer and M13 reverse primer for sequencing. •T3 promoter and T7 promoter for in vitro transcription. Trans1-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing. Storage At -20°C for 9 months Kit Content Component CT501-01 CT501-02 (20 rxns) (60 rxns) pEASY ®- T5 Zero Cloning Vector (10 ng/μl) 20 µl 3×20 µl Control Template (5 ng/μl) 5 µl 5 µl Control Primers (10 μM) 5 µl 5 µl M13 Forward Primer (10 μM) 50 µl 150 µl M13 Reverse Primer (10 μM) 50 µl 150 µl Please note: this kit does not include competent cells. References: Literature Journal IF Author Date Link Bisulfite-free, base-resolution analysis of 5-formylcytosine at the genome scale NATURE METHODS 25.328 Bo Xia, et al. State Key Laboratory of Protein and 2015 Sep The original link Enhanced Telomere Rejuvenation in Pluripotent Cells Reprogrammed via Nuclear Transfer Relative to Induced Pluripotent Stem Cells Cell Stem Cell 25.315 Rongrong Le et al. 2014 Jan The original link Replacement of Oct4 by Tet1 during iPSC Induction Reveals an Important Role of DNA Methylation and Hydroxymethylation in Reprogramming Cell Stem Cell 25.315 Yawei Gao, et al. 2013 Mar The original link Unique features of mutations revealed by sequentially reprogrammed induced pluripotent stem cells Nature Communications 11.329 Shuai Gao, et al. Ministry of Agriculture Key Labo 2015 Feb The original link High-throughput sequencing reveals the disruption of methylation of imprinted gene in induced pluripotent stem cells Cell Research 10.526 Gang Chang,et al. 2013 Dec The original link
