Description Rat Pancreatic Islets from Cell Biologics are isolated from the pancreas of Sprague–Dawley rat using Collagenase P and purified using Ficoll density gradient. The isolated islets are cultured in Cell Biologics’ Pancreatic Islet Culture Medium containing 5% FBS. Product Testing Rat Pancreatic Islets are tested for their viability using FDA/PI, purity using Dithizone staining, static insulin secretion in response to 14 mM glucose. Upon request, dynamic insulin secretion and calcium imaging in response to glucose and other stimulators can be performed. Suggested Medium Pancreatic islet culture medium /w Kit – 100 ML Catalog No. M2269 Laboratory Applications Rat Pancreatic Islets can be used for the assay of islet function, drug testing, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or others for desired research applications. Storage Cell Biologics ships fresh islet cells on at either room temperature or 4°C. On receipt, immediately transfer the cells to 37°C and 5% CO2 incubator. Documents RA-6232S Cell-Culture-Protocol-CB Disclaimer Appropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.
