Product Specifications: Item# 30070: Recombinant Dengue D2 Envelope Protein (E.coli) Concentration: See vial Mass/vial : See vial Diluent : 50mMTris, pH8, 10mM NaCl Purity : >95% Stabilizer : None Preservative: None Storage: -750C Physical State:Frozen Liquid Stability: At least 36 months at -750C. Applications: Elisa/Western Elisa, Diagnostics Description: Synthetic Dengue virus D2 Envelope DNA sequence, amino acid residues 1-428, expressed in the E.coli Expression System. Purification: This protein is purified by aqueous solvent extraction and preparative electrophoresis to >95% purity as determined by SDS-PAGE, reduced. Molecular Weight: approx. 49kD. Specificity: This protein shows strong reactivity with Dengue virus converted human serum antibodies as determined by Elisa and Western Blot assays. Useful for Dengue serum IgG/IgM detection. Application and Instructions for use Recommended concentrations for use are approximate values. Elisa is performed in 1-2ug/ml protein range for tittering Dengue virus converted human polyclonal antibodies. Concentrations if 1-2mg/ml (1-2ul/cm) are recommended for Lateral Flow Diagnostics.
