Product Specifications: Item# 30090: Recombinant Dengue Antigen D4 Envelope Protein (E.coli) Concentration: see vial Mass/vial: see vial Solids: 50mM NaCl,50mM Tris, pH8.5, 0.02%SDS Purity: >95% Stabilizer: None Preservative: None Storage: -750C Physical State: Frozen Liquid Stability: At least 36 months at -750C Applications: Elisa/Western Blot, Dengue Diagnostics Description: Synthetic Dengue virus D2 Envelope DNA sequence, amino acid residues 1-416, expressed as a fusion protein in the E.coli Expression System. Purification: This protein is purified by Ni affinity chromatography to >95% purity as determined by SDS-PAGE, reduced. Molecular Weight: approx. 75kD. Specificity: This protein binds to murine monoclonal antibodies – structural epitopes, and to Dengue virus converted human serum antibodies as determined by Elisa, Western Blots and by one-step lateral flow diagnostic testing. Application and Instructions for use: Recommended concentrations for use are approximate values. Elisa is performed in 1-2ug/ml protein range for tittering Dengue virus converted human polyclonal antibodies. Concentrations if 1-2mg/ml (1-2ul/cm) are recommended for Lateral Flow Diagnostics.
