FGF-2 (FGF-basic) Human, E. coli Recombinant Protein (154 aa)

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Description Our bioactive FGF-2 (FGF-basic) Human, E. coli Recombinant Protein (154 aa), also known as Fibroblast Growth Factor 2, is a single, non-glycosylated, polypeptide chain that contains 154 amino acids and has a 17.2 kDa molecular mass. The FGF-2 is purified by proprietary chromatographic techniques. Summary FGF-basic, a member of the fibroblast growth factor (FGF) family, is a non-glycosylated, heparin-binding growth factor and signaling protein encoded by the FGF2 gene. It binds to and exerts effects via specific fibroblast growth factor receptor (FGFR) proteins. Like other FGF family members, FGF-basic possesses broad mitogenic and cell survival activities, and is involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. Additionally, FGF-basic is a critical component of human embryonic stem cell (hESCs) culture medium; the growth factor is necessary for the cells to remain in an undifferentiated state. As a result, FGF-basic has been used to develop chemically-defined, serum-free and feeder-free hESCs culture mediums. CAS Registry No. 106096-93-9 Other Names Fibroblast Growth Factor-2, FGF2, FGF-2, FGF basic, FGF-b, Basic fibroblast growth factor, bFGF, Heparin-binding growth factor 2, HBGF-2. Amino Acid Sequence AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS. Source E. coli Formulation Sterile filtered white lyophilized (freeze-dried) powder. The protein was lyophilized from a concentrated (1 mg/ml) solution in 20mM Tris-HCl, pH7.4 and 1M NaCl. Purity Greater than 98.0% as determined by Analysis by SDS-PAGE. Biological Activity The ED50, calculated by the dose-dependent proliferation of murine balb/c 3T3 cells is Show more