Description GM-CSF is a monomeric protein of 127 amino acids with two glycosylation sites. The protein is synthesized as a precursor of 144 amino acids, which included a hydrophobic secretory signal sequence at the aminoterminal end. The sugar moiety is not required for the full spectrum of biological activities. Non-glycosylated and glycosylated GM-CSF show the same activities in vitro. Fully glycosylated GM-CSF is biologically more active in vivo than the non- glycosylated protein. This protein is secreted together with other factors by T cells and macrophages following cell activation by antigens or mitogens. Approximately 90% of the secreted colony stimulating activities are due to GM-CSF. This product is compatible with cell culture workflows associated with the Cell Biologics system, and is suitable for use in standard cytokine-supplemented media formulations commonly used with Cell Biologics primary cells and immune-related cell models. It can be used as a general GM-CSF source in Cell Biologics–based experimental setups, including immune cell culture and cytokine-dependent cell maintenance systems.
