Co-IP Optimized | Gentle Lysis | RUO | Interaction-Focused Extraction What is RIPA-Interact Cell Lysis Buffer (1X)? Atheris Bio™ RIPA-Interact Cell Lysis Buffer (1X) is a chemically defined lysis buffer designed for co-immunoprecipitation (Co-IP) and protein–protein interaction studies, where maintaining native interactions is important. The formulation uses a milder detergent system to reduce disruption of protein complexes during extraction, supporting recovery of interaction partners for downstream analysis. Key Benefits Designed for protein–protein interaction studies Gentle lysis conditions compared to standard RIPA buffers Supports recovery of complex-associated proteins Compatible with Co-IP and downstream Western blot workflows Ready-to-use format for workflow consistency Typical Applications Co-immunoprecipitation (Co-IP) Protein–protein interaction studies Signaling complex analysis Compatibility ✔ Mammalian cell lysates✔ Adherent and suspension cultures✔ Co-IP workflows✔ Western blot (downstream analysis) ⚠ Not recommended for: Strong denaturing lysis requirements Maximum membrane protein solubilization Enzyme activity assays Protein Quantification Compatibility Assay Method Compatibility Recommendation BCA Assay High ✅ Preferred Bradford (standard) Moderate ⚠ May vary Bradford (detergent-compatible) Moderate ✅ Acceptable Lowry Assay Poor ❌ Avoid A280 (UV) Poor ❌ Avoid Note: Reduced ionic detergent improves compatibility compared to standard RIPA, though optimization may still be required. Stability & Storage Condition Stability Unopened (2–8°C) 12 months Opened bottle Up to 8 weeks Room temperature Short-term handling only Freeze/thaw Not recommended Storage & Handling Store at 2–8°C Do not freeze Mix gently before use Use aseptic technique Quality Control Each lot is tested for: pH verification Visual clarity Functional performance Batch consistency Frequently Asked Questions How is RIPA-Interact different from RIPA-IP? RIPA-Interact™ uses gentler lysis conditions designed to help preserve protein interactions. Can it improve Co-IP results? It may improve recovery of interaction partners compared to harsher buffers. Which protein assay should I use? BCA assay is recommended for consistent results. Do I need inhibitors? Yes, recommended based on experimental needs. Is this product for clinical use? No. For Research Use Only (RUO). “Gentle lysis designed to help preserve protein interactions.”
