IP-Optimized | Balanced Lysis | RUO | Consistent Performance What is RIPA-IP Cell Lysis Buffer (1X)? Atheris Bio™ RIPA-IP Cell Lysis Buffer (1X) is a chemically defined lysis buffer developed for routine immunoprecipitation (IP) workflows, where consistent protein recovery and assay compatibility are critical. The formulation provides a balanced lysis environment, enabling efficient protein extraction while maintaining conditions suitable for antibody-based pull-down assays and downstream analysis. Key Benefits Designed for routine immunoprecipitation workflows Provides consistent protein extraction across samples Supports reliable downstream Western blot analysis Balanced detergent system for controlled lysis conditions Ready-to-use format to reduce preparation variability Typical Applications Immunoprecipitation (IP) Protein enrichment prior to Western blot Routine protein analysis workflows Compatibility ✔ Mammalian cell lysates✔ Adherent and suspension cultures✔ IP workflows✔ Western blot (downstream analysis) ⚠ Not recommended for: Weak or transient interaction studies (Co-IP) Enzyme activity assays Protein Quantification Compatibility Assay Method Compatibility Recommendation BCA Assay High ✅ Preferred Bradford (standard) Low ⚠ Limited accuracy Bradford (detergent-compatible) Moderate ✅ Acceptable Lowry Assay Poor ❌ Avoid A280 (UV) Poor ❌ Avoid Note: Ionic detergent content may affect dye-binding assays. Use appropriate standards and dilution. Stability & Storage Condition Stability Unopened (2–8°C) 12 months Opened bottle Up to 6 weeks Room temperature Short-term handling only Freeze/thaw Not recommended Storage & Handling Store at 2–8°C Do not freeze Mix gently before use Use aseptic technique Quality Control Each lot is tested for: pH verification Visual clarity Functional performance Batch consistency Frequently Asked Questions Can RIPA-IP™ be used for Co-IP? It may be suitable for strong interactions; gentler buffers are recommended for sensitive complexes. Is it compatible with Western blot? Yes, lysates are fully compatible with SDS-PAGE and Western blot workflows. Which protein assay should I use? BCA assay is recommended for reliable quantification. Do I need to add inhibitors? Yes, protease/phosphatase inhibitors are recommended depending on your application. Is this product for clinical use? No. For Research Use Only (RUO). “Balanced lysis for consistent immunoprecipitation.”
