SATB2 Polyclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: BS91209-50, BS91209-100 Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Swiss-Prot: Q9UPW6(Human) Q8VI24(Mouse) Host: Rabbit Reactivity: Human, Mouse, Rat Applications: WB, ICC/IF, IHC, FC All Applications: WB:1:500-1:1,000 ICC/IF:1:50-1:100 IHC:1:50-1:200 FC:1:50-1:100 Background: SATB2 (Special AT-rich sequence-binding protein 2) is a nuclear matrix protein that influences craniofacial formation mechanisms, such as jaw and palate development, and is part of a transcriptional network regulating skeletal development and osteoblast differentiation. Highly expressed in adult and fetal brain, SATB2 contains two CUT DNA-binding domains and one homeobox domain and is closely related to SATB1, a transcriptional repressor. SATB2 is thought to bind to matrix-attachment regions (MARs) and regulate MAR-dependent transcription of various genes, including HoxA2 and ATF4 (CREB-2), involved in skeletal development. Functioning as both a transcriptional activator and repressor, SATB2 can also act as a protein scaffold that can enhance the activity of other DNA-binding proteins. Defects in the gene encoding SATB2 are the cause of cleft palate manifested in conjunction with severe mental retardation. Purification and Purity: ProA affinity purified Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Specificity: SATB2 polyclonal antibody detects endogenous levels of SATB2 protein. Immunogen: Recombinant protein Conjugate: Unconjugated Modification: Unmodified Note: For research use only, not for use in diagnostic procedure. Extra Notes: Western blot analysis of SATB2 on THP-1 cell using anti-SATB2 antibody at 1/500 dilution., ICC staining SATB2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
