Juice™ scFv cell-free protein expression system is the only kit on the market specifically optimized for scFv expression. The mix can produce over ~1 mg of purified scFv constructs per milliliter of reaction in under 16 hours starting from optimized linear templates. It has been designed for T7-based protein expression and can generate proteins from standard inducible expression plasmids (e.g. pET15 and pET28) in the presence of 0.5-1mM IPTG. An important advantage of the Juice system is that it does not contain polyethylene glycol (PEG). PEG, which is often present in other commercially available cell-free systems, is a hydrophobic agent that can result in ion suppression at the ion source of a mass spectrometer and appears as repeating elements of 44 Da in the mass spectrum (Scheerlinck et al, 2015). Bulk cell-free is currently available in 1 mL aliquots (i.e. 334 μL of lysate per tube). If you require custom aliquots, please contact us. This kit can use plasmid or linear templates. Further resources: scFv Cell-Free Reagents Manual Store at -80°C. Catalog #: SC0001.B Publications: RNA binding proteins and glycoRNAs form domains on the cell surface for cell penetrating peptide entry A Rapid and Modular Nanobody Assay for Plug-and-Play Antigen Detection
