Trypsin-EDTA solution 0.5% (10X) does not contain phenol red and is a common reagent used in cell culture to detach adherent cells from the surface of culture vessels. This solution contains trypsin, an enzyme that breaks down cell-cell and cell-substrate adhesions by cleaving proteins, and ethylenediaminetetraacetic acid (EDTA), which helps neutralize trypsin activity and prevent cell clumping by chelating calcium and magnesium ions. The absence of phenol red in this solution makes it suitable for cells sensitive to phenol red or when phenol red may interfere with specific assays or experiments. Phenol red is a pH indicator commonly found in cell culture media. By using Trypsin-EDTA solution 0.5% (10X), researchers can effectively detach cells for passaging or experimentation while minimizing potential interference from phenol red, particularly when working with sensitive cell types or conducting assays where phenol red may affect the results. Disclaimer: Results may vary depending on specific cell lines, specifications, and experimental conditions. Consult with cell culture specialists and review comprehensive literature to tailor its use to your specific research needs.
