Trypsin EDTA Solution Catalog number: B2013036 Lot number: Batch Dependent Expiration Date: Batch dependent Amount: 25 mL Molecular Weight or Concentration: 23.4 kDa Supplied as: Solution Applications: molecular tool for various biochemical applications Storage: -20°C Keywords: Cell dissociation enzyme Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um. References Sonthalia S, Kachhawa D. Jodhpur Technique 2022 Jul 25. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2022 Jan–. Huh MI, Lee KP, Kim J, Yi S, Park BU, Kim HK. Generation of Femtosecond Laser-Cut Decellularized Corneal Lenticule Using Hypotonic Trypsin-EDTA Solution for Corneal Tissue Engineering J Ophthalmol. 2018 Apr 1;2018:2590536. Lindstad RI, Sylte I, Mikalsen SO, Seglen PO, Berg E, Winberg JO. Pancreatic trypsin activates human promatrix metalloproteinase-2 J Mol Biol. 2005 Jul 22;350(4):682-98. Giraldo-Gomez DM, Leon-Mancilla B, Del Prado-Audelo ML, Sotres-Vega A, Villalba-Caloca J, Garciadiego-Cazares D, Piña-Barba MC. Trypsin as enhancement in cyclical tracheal decellularization: Morphological and biophysical characterization Mater Sci Eng C Mater Biol Appl. 2016 Feb;59:930-937. Kawamura K, Nishitsuji K, Shoguchi E, Fujiwara S, Satoh N. Establishing Sustainable Cell Lines of a Coral, Acropora tenuis Mar Biotechnol (NY). 2021 Jun;23(3):373-388. Wu J, Luan M, Zhao J. Trypsin immobilization by direct adsorption on metal ion chelated macroporous chitosan-silica gel beads Int J Biol Macromol. 2006 Nov 15;39(4-5):185-91. Curr Protoc Cytom. 2001 May;Appendix 2:Appendix 2A. doi: 10.1002/0471142956.cya02as09. Common stock solutions, buffers, and media Curr Protoc Cytom Mattioli-Belmonte M, Orsini G, Giuliodori F, DI Cristoforo A, Procaccini M, Mengucci P, Putignano A. Evaluation of an automated system for root canal irrigation: a scanning electron microscopy study Dent Mater J. 2012;31(6):969-74. Zhu YT, Tighe S, Chen SL, John T, Kao WY, Tseng SC. Engineering of Human Corneal Endothelial Grafts Curr Ophthalmol Rep. 2015 Sep;3(3):207-217.
