Terminal Deoxynucleotidyl Transferase (TdT) is a template-independent DNA polymerase that catalyzes the repetitive addition of deoxynucleotides to the 3'-hydroxyl termini of oligonucleotides, single-stranded, and double-stranded DNA. The TdT reaction requires short sequences of at least three bases to act as primers. When using RNA as a substrate, TdT activity is highly dependent on the tertiary structure and nucleotide composition at the 3’-end of the acceptor RNA. In general, TdT exhibits lower efficiency with RNA templates compared to DNA templates. Specification Cat. No. 10302ES76 / 10302ES86 / 10302ES99 / 10302ES98 Size 500 U / 2,500 U / 180,000 U / 333,000 U Concentration 20 U/µL Source Recombinant expression in E.coli Unit Definition One unit (U) is defined as the amount of enzyme required to incorporate 1 nmol of deoxyribonucleotide into polydeoxynucleotides in 60 minutes at 37°C. Inhibitors Metal chelators, ammonium, chloride, iodide, and phosphate ions. Inactivation Add EDTA and heat at 70°C for 10 min. Components Components No. Name 10302ES76 10302ES86 10302ES99 10302ES98 10302-A Terminal Deoxynucleotidyl Transferase (20 U/µL) 25 μL 125 μL 9 mL 16.65 mL 10302-B 5× Reaction Buffer 400 μL 2×1 mL 40 mL 74 mL Storage This product should be stored at -25~-15℃ for 1 year. Application DNA End Labeling; TUNEL Assay for Apoptosis Detection; Next-Generation Sequencing (NGS) Library Preparation; DNA Tail Extension and Modification; Molecular Diagnostics and Biosensing(CRISPR or DNAzyme biosensors). Figures 1. High purity — no nicking enzyme detectable, no endonuclease detected. Figure 1. Residual nicking enzyme and exonuclease activities were tested in two independent batches, and no residues were detected. Documents: Safety Data Sheet 10302_MSDS_HB20260605 Manuals 10302_Manual_20260605
