Proteinase K is a broad-specificity serine protease with a relative molecular weight of approximately 29.3 kDa. It cleaves peptide bonds at the carboxyl side of aliphatic and aromatic amino acids. Proteinase K has wide applications, including preparation of high-molecular-weight chromosomal DNA for pulsed-field gel electrophoresis, Western blotting (protein immunoblotting), and removal of nucleases during DNA and RNA preparations. Denaturants such as SDS (1%) can enhance its activity. The typical working concentration is 50–100 µg/mL, with the exact concentration determined based on factors such as the presence of SDS or urea in the buffer, pH, and temperature. This product is a low-residual Proteinase K, which maintains the enzyme's inherent properties while featuring significantly reduced background microbial residues, ensuring greater accuracy in downstream detection applications. Features Derived from Tritirachium album (also known as Parengyodontium album or Limber strain in some references); Specific activity: ≥800 U/mL; Protein concentration: ≥20 mg/mL; Free of RNase and DNase contamination; Stable activity in the presence of urea and SDS; Active over a broad pH range (pH 4.5–12.5), with optimal activity between pH 7.0–12.0; Reaction conditions: Incubate in reaction buffer at 37°C–65°C for 1 hour. Recommended temperature: 65°C; Unit definition: One unit is the amount of enzyme required to hydrolyze casein to release 1 µmol of L-tyrosine per minute under defined conditions; Inactivation method: Add PMSF or DFIP inhibitor to the reaction system for complete inactivation. Applications Nucleic Acid Extraction & Purification Remove endogenous nucleases during the preparation of DNA and RNA Prepare tissue sections for in - situ hybridization. Specifications Cat.No. 10410ES01 / 10410ES05 / 10410ES60 /10410ES70 Size 1 mL /5 mL/ 100 mL /1000 mL Shipping and Storage This product should be stored at 2~8℃ for 2 years.For longer storage, it can be stored at -25~-15℃. Documents: 10410-MSDS-HB2603181 10410-Manual-Ver.EN20250617 COA Related Blog Low-DNA Proteinase K: Eliminate Background Noise in Pathogen Detection
