Proteinase K is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids with a relative molecular weight of 29.3 kDa.The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide .Proteinase K is widely used for the preparation of chromosomal DNA , and removal of DNases and RNases when isolating DNA and RNA from tissues or cell lines.Denaturants such as SDS (1%) can increase their activity.It is commonly used at working concentrations of 50-100 μg/mL. Features Enzyme activity ≥30 U/mg No RNase residue No DNase residue Applications Nucleic Acid Extraction & Purification Remove endogenous nucleases during the preparation of DNA and RNA Prepare tissue sections for in - situ hybridization. Specifications Cat.No. 10412ES03 / 10412ES10 / 10412ES25 /10412ES76 Size 1 mL /10 mL/ 25 mL / 500 mL Shipping and Storage This product should be stored at 2~8℃ for 2 years.For longer storage, it can be stored at -25~-15℃. Product Properties Source Yeast CAS NO. 39450-01-6 Molecular Weight 29 kD Units/mg ≥30 U/mg Appearance Liquid Purity ≥95% SDS-PAGE DNase Contamination NO RNase Contamination NO Unit Definition At 37°C, pH 7.5, every minute released 1 μM tyrosine required Proteinase K is defined as 1 U Documents: Safety Data Sheet 10412ES-MSDS-HB240223.PDF Manuals 10412_Manual_Ver.EN20250617_EN.pdf
