Proteinase K - Solution, 20 mg/ml, sterile, ready-to-use, for biochemistry and moleculr biology, Density (D) 1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. -20 °C, CAS No. 39450-01-6, EC No. 254-457-8 Proteinase K (from Tritirachium album) is a non-specific protease of the serine protease family. Proteinase K is used for the cleavage of proteins in nucleic acid preparations. It is mainly used in nucleic acid purification or for the removal of nucleases.Proteinase K is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS. Directions for use Foreign activity: RNAse and DNAse not detectableOptimum temperature: +65 °C.Activity at +65 °C is ca. 12 x higher than at +25 °C. Over +65 °C, inactivation due to denaturation.Activators: Denaturating agents like SDS (0,5-1 %), urea.Inhibitors: Inhibition with Hg2+-ions, DFP, PMSF and phenol. Not inhibited by EDTA, sulfhydryl reagents and trypsin or chymotryps ininhibitors.Stability: pH 4.0-12.5. pH optimum: 8,0.Also stable even when denaturing agents, e.g. SDS and urea are present.Stabilisers: Ca2+-ions (1-5 mM) prevent autolysis. Proteinase K (from Tritirachium album) is a non-specific protease of the serine protease family. Proteinase K is used for the cleavage of proteins in nucleic acid preparations. It is mainly used in nucleic acid purification or for the removal of nucleases. Sterile ready-to-use solution Activity ≥ 800 mAnsonU/ ml Stable over a wide pH range: 4.0–12.5 Active at high temperatures and denaturing conditions Technical Information Additional info Source: Tritirachium album PDF Technical Documentation (PDF)
