The RNase inhibitor (RNase inhibitor) is a specific ribonuclease (RNase) inhibitor that exists in human placenta. The principle of specific inactivation of RNase is: non-covalent bond with RNase to form a complex, resulting in RNase is inactivated. The product is a purified recombinant murine RNase inhibitor expressed in E.coli in soluble form. It can extensively inhibit various RNases (RNase A, B, C). According to RT-PCR and RT-qPCR, this product can be compatible with HifairTM V Reverse Transcriptase (Cat#11300) and various DNA Polymerases. Compared to human-derived RNase inhibitors, the murine version lacks two oxidation-sensitive cysteines, giving it higher antioxidant stability and making it better suited for DTT-sensitive applications like qPCR. The product can be applied to 1st strand synthesis of cDNA, polysome isolation, in vitro reverse transcription and in vitro cell-free translation system. This glycerol-free formulation is specially designed for lyophilization. Features Highly effective RNase inhibition activity Excellent high temperature tolerance Components Name 10703(2 KU) 10703(10 KU) 10703(80 KU) 10703(100 KU) 10703(200 KU) 10703(1000 KU) Murine RNase Inhibitor (200 U/µL, Glycerol-free) 10 μL 50 μL 400 μL 500 μL 1 mL 5 mL Shipping and Storage This product should be stored at 2-8°C for 6 months. Application This product is ideal for preventing RNA degradation in any workflow where RNase contamination may occur, including: cDNA synthesis & RT-PCR – Compatible with both PCR and qPCR systems. In vitro transcription/translation – Protects RNA in systems such as viral replication assays. RNA isolation & purification – Maintains RNA integrity throughout the process. RNase protection assays – Ensures accurate and reliable results. Figures 1.Highly effective RNase inhibition activity Figure 1. RNase Inhibitor Effectively Inhibits RNase Activity 2.Compatible with various lyophilized forms for flexible use Figure 2. Freeze-Dried Product Forms Using Yeasen's RNA lyophilizable reagent (Cat#16645ES), which contains Murine RNase Inhibitor (200 U/µL, Glycerol-free) (Cat#10703), both in situ freeze-drying and freeze-dried microspheres were successfully prepared. The in situ freeze-dried product showed a smooth surface with no collapse or tube gap. The microspheres were uniform, plump, and smooth. 3.No residual exonuclease, nicking enzyme, or RNase. Figure 3. No detectable residues of exonuclease, nicking enzyme, or RNase. N* stands for negative control; 1, 2 and 3 are 3 batches. Documents: Safety Data Sheet 10703_MSDS_HB250619_EN.pdf Manuals 10703_Manual_Ver.EN20250707.pdf Related blog: How to produce an excellent Glycerol-free molecular enzyme?
