Hieff NGS™ EvoMax RNA Library Prep Kit (Strand-specific) _ 12340ES

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Hieff NGS™ EvoMax RNA Library Prep Kit (Strand-specific) is a premixed, actinomycin D–free, strand-specific total RNA sequencing library preparation kit compatible with both Illumi na and MGI platforms. Available in two formats—tube and sealing kit—this kit offers convenience for both automated liquid handling systems and manual operation. The kit includes reagents for RNA fragmentation, reverse transcription, strand-specific double-stranded cDNA synthesis, and library amplification. It can be combined with mRNA purification or rRNA removal kits for mRNA or lncRNA studies. By optimizing the reverse transcription step, this kit achieves high strand specificity without the use of actinomycin D, enhancing user safety. All reagents undergo stringent quality control and functional validation to ensure maximum stability and reproducibility in library preparation. Features Streamlined 5-Tube Workflow (Saves Up to 2 Hours): Meticulously optimized enzyme and buffer master mixes simplify handling, accelerate hands-on time, and significantly minimize manual pipetting errors. The World’s First Actinomycin D-free RNA Library Prep Kit: Eliminates cytotoxic Actinomycin D for safer lab handling, and requires no light protection to enable seamless, high-throughput automated workflows. High Exon Coverage Uniformity & Improved Strand Specificity: Driven by engineered M-MLV and T4 DNA ligases (via rational design & directed evolution) to ensure exceptional transcript coverage, strand specificity, and high yield. Available in Tube & 96-Well Pre-filled Plate Formats: Choose flexible tubes for manual prep, or automation-ready pre-filled plates for effortless "peel-and-run" high-throughput runs. Trusted Brand & Market-Proven Scale: Delivering >4 million DNA and >2 million RNA library prep reactions per year, supported by long-tested supply chains and QC systems to reliably empower routine high-throughput sequencing. Full-Service On-Site Support — Seamless & Effortless Switching: Enjoy hands-on, head-to-head validation and free automated script setup by local technical team—switch kits seamlessly with minimal time and effort. Components Components No. Name 12340ES08 12340ES24 12340ES96 12340ES97 (Tube, Automated 96T) 12340-A Frag/Prime Buffer 150 μL 450 μL 2×900 μL 2×1064 μL 12340-B 1st Reaction Module 2.0 64 μL 192 μL 768 μL 960 μL 12340-C 2nd Reaction Module(dUTP) 280 μL 840 μL 3×1120 μL 3×1280 μL 12340-D Ligation Reaction Module 280 μL 840 μL 3×1120 μL 3×1280 μL 12340-E 2×Super CanaceTM II High-Fidelity Mix 200 μL 600 μL 2×1200 μL 2×1360 μL * Primer Mix* / / / [Note]: * The notation indicates that the component is not included in the kit. Primer mix is required if the complete adapters are used, otherwise it isn’t. This kit is compatible with Illumina and MGI platforms, but need additional primer mix (Cat # 13334 Primer Mix for MGI and Cat # 13335 Primer Mix for Illumina) for Illumina or MGI platform if the complete adapters are used. ** See the following diagram for the layout of plate reagent group. Storage This product should be stored at -25~-15℃ for one year.. Application RNA library Preparation; Transcriptome profiling and gene expression analysis; mRNA sequencing for coding RNA studies; lncRNA (long non-coding RNA) research; Small RNA sequencing (if applicable); Differential gene expression analysis; RNA biomarker discovery; RNA variant and fusion detection. Figures 1.Simplified Product Format Figure 1. Simplied components of EvoMax RNA Library Prep Kit. 2.High Exon Coverage Uniformity Figure 2. Yeasen Kit show high exon coverage uniformity compared to NEB kit. The high GC exon were effectively detected. 3.High-Yield, Flexible RNA Library Preparation Figure 3. Comparison of RNA Library Yields. (A, B) Strand-specific mRNA libraries from human, wheat, and mouse RNA (200 ng, 500 ng) using Hieff NGS™ EvoMax RNA Library Prep Kit (Strand-specific) (Cat# 12340ES) and mRNA Isolation Master Kit (Cat#12629) showed higher yield than Supplier N*. (A) Qubit quantification. (B) qPCR quantification. (C) Strand-specific LncRNA libraries from mouse RNA (200 ng, 500 ng) using the NGS Premix RNA Library Prep Kit with rRNA Depletion Kit (Human/Mouse/Rat, Cat#12726) showed higher yield than Supplier K* (qPCR). (D) Strand-specific LncRNA libraries from plant RNA (Arabidopsis, wheat, soybean leaves, 200 ng, 500 ng) using the NGS Premix RNA Library Prep Kit with rRNA Depletion Kit (Plant, Cat#12254) showed higher yield than Supplier K* (qPCR). 4.Higher Strand Specificity, More Transcripts Figure 4. Comparison of mRNA Library Strand Specificity and Transcript Composition. Figure 4. Comparison of mRNA Library Strand Specificity and Transcript Composition. (A) Human mRNA libraries prepared from 200 ng and 500 ng input demonstrated strand specificity of 99.4% and 99.3%(Cat#12340), compared with 99.0% and 99.2% for Supplier N*, respectively. (B) Transcript composition analysis showed that 12340ES libraries consistently detected more transcripts. With 200 ng input, 12340 detected 89.3% of coding + UTR transcripts versus 84.9% for Supplier N*. At 500 ng input, 12340ES detected 89.6% compared with 85.1% for Supplier N*. 5.Automation-Friendly Figure 5. Comparison of Library Yields Between Automated and Manual Prep. (A).Four RNA standard samples (D5, D6, F7, M8) were used (3 replicates each) with the Hieff NGS™ EvoMax RNA Library Prep Kit (Strand-specific) (Cat# 12340ES) and mRNA Isolation Master Kit (Cat#12629). Libraries were prepared using the automated platform and manual workflow. Both automated and manual preps showed highly uniform library yields across all samples. (B). Accuracy of gene expression quantification. Fold-change analysis of RNA Library Prep Kit(Cat#12340)and mRNA Isolation Master Kit (Cat#12629)against the RNA standard dataset showed high consistency (Pearson r2 > 0.94). 6.Strong Stability Figure 6. Stability Evaluation. (A) Accelerated stability was tested at 4°C and 25°C, with −20 °C storage as the control. (B) Real-time stability was monitored for up to 12 months. 7.RNA Library prep for different quality FFPE samples For severely degraded FFPE RNA (DV 200 Show more