Anti-rabbit Nanobody-Tn5 is a recombinant Tn5 transposase fused with nanobody which can specific bind with primary antibodies from rabbit. Product Information Catalog No. 12594ES04/12594ES12/12594ES48 specifications 4T/ 12T/ 48T Product Components Component name 12594ES04 12594ES12 12594ES48 12594-A Anti-rabbit Tn5 ( 0.65 mg/mL) 4 μL 12 μL 48 μL 12594-B Annealing Buffer 1 mL 1 mL 1mL 12594-C Assemble Buffer 4 μL 12 μL 48 μL Shipping and Storage Shipped on dry ice. This product should be stored at -85~-65℃ for one year. Figures H3K4me1: H3K4me3 H3K9me3 H3K27ac H3K27me3 H3K27me3 Control: Conventional pA/G-Tn5, Cut&Tag Multi Cut&Tag: Nanobody Tn5 Instruction Take high-throughput sequencing library preparation as an example, be sure to read the instructions carefully before use. 1. Adapter preparation 1) Reference oligo name and sequence of Illumina platform: Oligo A-1：5'-AATGATACGGCGACCACCGAGATCTACACATAAGGCTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-3' Oligo A-2：5'-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-3′ Oligo B：5'-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-3′Oligo C：5'-phos-CTGTCTCTTATACACATCT-3' 2) Dissolve the Oligo A, Oligo B, and Oligo C to 200 μM. 3) The following reaction systems are formulated respectively: component Reaction 1 Oligo B （200 μM） 10 μL Oligo C （200 μM） 10 μL total 20 μL component Reaction 2 Oligo A-1 or A-2 （200 μM） 10 μL Oligo C （200 μM） 10 μL total 20 μL 4) Mix reaction 1 and 2 together, and briefly centrifuge to bring the solution back to the bottom of the tube. Place in the PCR instrument, set up the following program and run it: hot lid at 105°C,and then heat at 94℃ for 2 min. After the time was up, the reaction procedure was stopped without opening the hot lid.The PCR tube was naturally cooled in the PCR instrument for 2 h, and then placed at 4℃ for 5 min. 5) After the reaction, mix the equal volumes of Reaction 1 and Reaction 2. Named ad Adapter Mix and stored from – 25 to – 15℃. 2. for transposon generation 1) To configure the following reaction system: component volume (μL) Anti-rabbit Tn5 10 Adapter mix (50 μM) 0.5 Assemble Buffer 0.5 2) Reaction conditions: use the pipette to gently blow and mix well. Perform the reaction at 25℃ for 1 h (hot lid off). The products of reaction can be directly applied to the library preparation or stored from -25 to -15℃. Notes 1. Transposase is temperature-sensitive, so it is recommended to complete the adapter embedding as soon as possible. The generated transposon can be stored at-25~ -15℃, with a validity period of 1 year. 2. This product is used for scientific research purposes only. 3. For your safety and health, please wear laboratory clothes and wear disposable gloves. Documents: Safety Data Sheet 12594_MSDS_HB251216_EN.PDF Manuals 12594_Manual_HB20260602
