1×dsDNA HS Assay Kit is a rapid, highly sensitive and accurate fluorescent quantitative detection kit for double-stranded DNA (dsDNA). This kit is highly selective for dsDNA and has good linearity in the range of 0.2 ng-100 ng, the quantitation range is between 10 pg/μL to 100 ng/μL. This kit is easy to operate, providing a ready-to-use working solution that enables simple dsDNA sample quantification on Qubit Fluorometer or Fluorescence Microplate Reader. It is ideal choice for NGS large-scale DNA sample quantification (such as input DNA quantification, DNA library quantification, etc.). This kit is well tolerated to common contaminants such as proteins and salts. Features Fast: Ready-to-use premixed solution for easy handling; results available in just 5 minutes. Sensitive: Accurately quantifies samples ranging from 0.2 to 100 ng. Accurate: Minimal deviation compared to competing products, with all errors controlled within 10% and low batch-to-batch variation. Stable: Consistent performance across different production batches. Components Components No. Name Concentration 12642ES60 (100 T) 12642ES76 (500 T) 12642-A 1×dsDNA HS Working Solution 1×Concentrate in DMSO 50 mL 250 mL 12642-B dsDNA Standard 1 0 ng/μL in TE buffer 1 mL 5×1 mL 12642-C dsDNA Standard 2 10 ng/μL in TE buffer 1 mL 5×1 mL Shipping and Storage This product should be stored at 2~8℃ away from light for 12 months. Application dsDNA quantification; DNA concentration determination Figures 1. Easy-to-use and streamlined quantification workflow Figure 1. DNA Quantification Workflow 2. Good linearity: 12642 has good linearity in the range of 0.2-100 ng. Figure 2. Linear Relationship Test Linear quantification of 11 dsDNA concentrations (0.5–10 ng/μL) using 12642. Fluorescence was measured with Qubit Fluorometer 3.0. Results show good linearity across 0.2–100 ng total dsDNA. 3. Dye binding specificity Figure 3. Specificity test Test Scheme: DNA standard S2 (1–100 ng gradient: 1, 5, 10, 20, 40, 60, 80, 100 ng), along with equal-mass samples of ssDNA, RNA, dsDNA+ssDNA mix, and dsDNA+RNA mix were tested. Conclusion: Adding equal amounts of ssDNA slightly increases the 12642 reading; error
