Description 1×dsDNA HS Assay Kit is a rapid, highly sensitive and accurate fluorescent quantitative detection kit for double-stranded DNA (dsDNA). This kit is highly selective for dsDNA and has good linearity in the range of 0.2 ng-100 ng, the quantitation range is between 10 pg/μL to 100 ng/μL. This kit is easy to operate, providing a ready-to-use working solution that enables simple dsDNA sample quantification on Qubit Fluorometer or Fluorescence Microplate Reader. It is ideal choice for NGS large-scale DNA sample quantification (such as input DNA quantification, DNA library quantification, etc.). This kit is well tolerated to common contaminants such as proteins and salts. Features High sensitive: the concentrations ranging from 10 pg/µ l to 100 ng/µL of dsDNA can be accurately quantified; High specificity: this product is highly selective to dsDNA and is not affected by RNA, has good tolerance to some conventional pollutants, such as salt, free nucleotide, protein, solvent, detergent, etc; Ready to use: 1x working solution, just add the dsDNA sample to be tested and test it with Qubit fluorometer or Fluorescence Microplate Reader at room temperature Applications dsDNA quantification with Qubit or Microplate Reader NGS library quantification Specifications Assay dsDNA Quantitation Excitation/Emission 480/520 For Use With (Equipment) Qubit Fluorometer, Microplate Reader No. of Reactions 100T/500T Product Line Qubit Quantitation Quantitation Range 10 pg/μL to 100 ng/μL Sample Volume 1 μL to 20 μL Detection Method Fluorescent Components Components No. Name Concentration 12642ES60 (100T) 12642ES76 (500T) 12642-A 1×dsDNA HS Working Solution 1×concentrate in DMSO 50 mL 250 mL 12642-B dsDNA Standard 1 0 ng/μL in TE buffer 1 mL 5×1 mL 12642-C dsDNA Standard 2 10 ng/μL in TE buffer 1 mL 5×1 mL Shipping and Storage All the components are shipped with ice pack and can be stored at 2-8℃ away from light for one year. Documents: Manuals 12642_Manual_HB221028_ EN.pdf Safety Data Sheet 12642-COMPONENT A-MSDS-HB220420.pdf 12642-COMPONENT B-MSDS-HB220420.pdf 12642-COMPONENT C-MSDS-HB220420.pdf
