T4 Polynucleotide Kinase (T4 PNK) is a 5'-hydroxyl kinase that facilitates the transfer of the γ-phosphate from ATP to the 5'-hydroxyl termini of oligo- or polynucleotide chains, including double-stranded and single-stranded DNA or RNA, as well as to 3'-monophosphate nucleotides. This transfer is reversible. T4 PNK also possesses 3'-phosphatase activity, which dephosphorylates 3'-phosphorylated oligonucleotide termini, deoxy 3'-monophosphate nucleotides, and deoxy 3'-diphosphate nucleotides. In the presence of ADP, T4 PNK can act as a 5'-phosphatase, promoting the exchange of 5'-phosphate groups on 5'-phosphorylated oligo-or polynucleotides with ATP. This enzyme is widely used for the construction of DNA libraries and for end labeling to generate probes. Applications Phosphorylation of the 5' ends of DNA or RNA for subsequent ligation Terminal labeling of DNA or RNA to serve as probes and for sequencing purposes 5' phosphorylation of mononucleotides with a phosphorylated 3' end to prepare pNp substrates for attachment to the 3' ends of DNA or RNA Labeling the 5' ends of oligonucleotides that possess a 3' phosphate group Specifications Source Bacteriophage T4 Molecular weight 36.78 kDa Concentration 10 U/μL Unit definition One unit is defined as the the amount required to catalyze the incorporation of 1 nmol of [γ-32P] ATP into a reaction within 30 minutes at 37°C Components Components No. Name 12902ES76 12902ES86 12902ES92 12902-A T4 polynucleotide Kinase(10 U/μL) 50 μL 250 μL 1 mL 12902-B 10×T4 PNK Buffer 500 μL 1 mL 2×1 mL [Note]: The 10×T4 PNK Buffer does not contain ATP; customers need to add it themselves, with a reference final concentration of 1 mM, or use the T4 DNA ligase buffer. Shipping and Storage This product should be stored at -25 ~ -15℃ for 1 year. Documents: Safety Data Sheet 12902_MSDS_Ver.EN20241210.pdf Manuals 12902_Manual_Ver.EN20250729.pdf
