The Hieff NGS™ ds-cDNA Synthesis Kit is a cDNA synthesis kit for RNA sequencing libraries on the Illumina™ /MGI™ sequencing platforms, containing efficient RNA reverse transcription reagents and conventional ds-cDNA synthesis reagents. It can be connected to the Hieff NGS™ OnePot Flash DNA Library Prep Kit (Yeasen Cat#12316ES) for subsequent library construction. All reagents provided in this kit have undergone strict quality control and functional validation, ensuring the stability and reproducibility of library construction to the greatest extent. Features Fast: Rapid cDNA Synthesis. No purification of the second-strand product. cDNA synthesis only takes 53 minutes. Compatible: Compatible with 100 pg - 500 ng total RNA. High-quality: Upgraded reverse transcription module, excellent pathogen detection, and strict background bacteria quality control. Applications · Double-stranded cDNA Synthesis · Metatranscriptome Detection · Pathogen RNA library Prep Specifications Cat.No. 13488ES08 / 13488ES24 / 13488ES96 Size 8 T / 24 T / 96 T Components Components No. Name 13488ES08 13488ES24 13488ES96 13488-A Random Primer 8 μL 24 μL 212 μL 13488-B 1st Reaction Buffer 64 μL 192 μL 1267 μL 13488-C 1st Strand Enzyme Mix 16 μL 48 μL 320 μL 13488-D 2nd Reaction Buffer 56 μL 168 μL 1034 μL 13488-E 2nd Strand Enzyme Mix 24 μL 72 μL 473 μL Shipping and Storage This product should be stored at -25~-15℃ for 1 year. Figures RNA input 100 pg-100 ng RNA: 100 pg-100 ng ds-cDNA Synthesis ds-cDNA Synthesis (Cat#13488ES) ds-cDNA Synthesis with gDNA digester (Cat#13488ES) ds-cDNA Synthesis time 53 min 92 min Library Prep Flash DNA Library Prep (Cat#12316ES) Flash DNA Library Prep (Cat#12316ES) Library Prep Time 75 min 75 min Species compatibility RNA (prefer) or DNA/RNA mixture RNA or DNA/RNA mixture 1. Comparison of RNA virus detection between the conventional RNA library construction approach and the reverse-transcription-then-DNA library construction approach Figure 1: Comparison of RNA virus detection across different library construction approaches Reverse transcription of RNA viruses combined with depletion of residual host rRNA effectively improves the detection rate of RNA viruses. 2. Performance demonstration of the DNA/RNA co-library construction approach using reference standards Figure 2: Performance of DNA/RNA co-library construction DNA/RNA co-library construction was performed using 10 ng of human RNA standard, 10 ng of calf genomic DNA (gDNA), and 5 ng of human gDNA spiked with Zymo at a 1/1000 ratio. The Yeasen kit demonstrated superior performance. Documents: Safety Data Sheet 13488_MSDS_HB240223 Manuals 13488_Manual_HB20260712
