The Hieff NGS™ C37P3 ds-cDNA Synthesis Kit (gDNA digester plus) is a cDNA synthesis kit for RNA sequencing libraries for the Illumina™/MGI™ sequencing platforms, which includes gDNA removal reagents, RNA reverse transcription reagents, and ds-cDNA synthesis reagents. All reagents provided in this kit have undergone strict quality control and functional validation to ensure the stability and reproducibility of library construction to the greatest extent. Features Fast: Rapid cDNA Synthesis. No purification of the second-strand product. gDNA digester and cDNA synthesis only takes 92 minutes. Compatible: Compatible with 100 pg - 10 ng total RNA, as well as nucleic acids co-extracted from DNA and RNA. High-quality: Upgraded reverse transcription module, excellent pathogen detection, and strict background bacteria quality control. Applications Double-stranded cDNA Synthesis Metatranscriptome Detection Pathogen Co-library Prep Specifications Cat.No. 13501ES08 / 13501ES24 / 13501ES96 Size 8 T / 24 T / 96 T Components Components No. Name 13501ES08 13501ES24 13501ES96 13501-A DNaseⅠBuffer 16 μL 48 μL 192 μL 13501-B DNase I Mix 16 μL 48 μL 192 μL 13501-C 1st Reaction Buffer 48 μL 144 μL 580 μL 13501-D 1st Strand Enzyme Mix 16 μL 48 μL 192 μL 13501-E 2nd Reaction Buffer 56 μL 168 μL 672 μL 13501-F 2nd Strand Enzyme Mix 24 μL 72 μL 288 μL Shipping and Storage This product should be stored at -25~-15℃ for 1 year. Figures RNA input 100 pg-100 ng RNA: 100 pg-100 ng ds-cDNA Synthesis ds-cDNA Synthesis (Cat#13488ES) ds-cDNA Synthesis with gDNA digester (Cat#13488ES) ds-cDNA Synthesis time 53 min 92 min Library Prep Flash DNA Library Prep (Cat#12316ES) Flash DNA Library Prep (Cat#12316ES) Library Prep Time 75 min 75 min Species compatibility RNA (prefer) or DNA/RNA mixture RNA or DNA/RNA mixture Documents: Safety Data Sheet 13501_MSDS_HB240223 Manuals 13501_Manual_HB20260712
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