Hieff Unicon™ Hotstart J-Taq DNA Polymerase, Glycerol-free (40 U/μL) is a hot-start DNA polymerase. This product utilizes antibody-mediated inhibition, providing high amplification sensitivity and specificity. The inhibitory antibody is completely inactivated and the DNA polymerase activity is fully released after a 30-second heat activation step at the initial denaturation temperature. Using this hot-start Taq enzyme effectively suppresses non-specific amplification caused by primer mis-annealing. This glycerol-free formulation is specifically designed for lyophilized reagent preparation. Features Rapid Activation: Taq polymerase achieves full activity after a quick 30 s incubation at 95 °C, ensuring immediate and efficient amplification. High Sensitivity & Specificity: Provides strong amplification performance even with low template input, minimizing non-specific products. Broad Instrument Compatibility: Works seamlessly with a wide range of standard PCR and real-time PCR platforms. Lyophilization-Ready: Formulation supports freeze-drying, enabling long-term storage, reduced shipping costs, and simplified reagent handling. Components Name 14326ES02 14326ES20 14326ES30 Hotstart J-Taq DNA Polymerase, Glycerol-free ( 40 U/μL) 50 μL 500 μL 2×1 mL Storage This product should be stored at -25~-15℃ for 1 year. Application Gene Expression Analysis; Low-Copy Target Amplification; Lyophilized PCR/qPCR. Figures 1. Consistent Performance & Strong Stability Figure 1. Stability of Yeasen glycerol-free enzyme (14326ES). The glycerol-free enzyme showed the same amplification performance as the glycerol-containing enzyme across COVID-19 pseudovirus templates (105–103 copies/mL). After 54 days at 37 °C, its performance remained unchanged, confirming excellent thermal stability. Documents: Safety Data Sheet 14326_MSDS_HB251010_EN.PDF Manuals 14326_Manual_Ver.EN20251127.pdf
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